Hotspot

rps16-trnQ-CAA

LSC · 6073-6490 · 418 bp

Back to Crucihimalaya

Pi 0.0211
MarkerSeek score 65.50
Primer available yes
Label rps16-trnQ-CAA

Feature Metadata

label_name
rps16-trnQ-CAA
feature_type
igs
region
LSC
length_bp
418
variable_sites
17
pi
0.0211
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
65.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-CAA_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 478-495 bp 1.0 83.2
rps16-trnQ-CAA_p2 CCTTCAATTCAAGTCGCACG GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.8 58.1 488-505 bp 1.0 82.3
rps16-trnQ-CAA_p3 CCACAGCAGATCATGTCCTT GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.6 58.1 504-521 bp 1.0 80.7
rps16-trnQ-CAA_p4 TCCACAGCAGATCATGTCCT GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.4 58.1 505-522 bp 1.0 80.7
rps16-trnQ-CAA_p5 ATCCACAGCAGATCATGTCC GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.4 58.1 506-523 bp 1.0 79.0