Hotspot

trnH-CAC-psbA

LSC · 77-354 · 278 bp

Back to Crucihimalaya

Pi 0.0546

MarkerSeek score 74.90

Primer available yes

Label trnH-CAC-psbA

Feature Metadata

label_name: trnH-CAC-psbA
feature_type: igs
region: LSC
length_bp: 278
variable_sites: 30
pi: 0.0546
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 74.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-CAC-psbA_p1	CTGCCTTAATCCACTTGGCT	ACTGAGTTTCCGTCTGGGTA	50.0	50.0	57.9	58.0	973-1021 bp	1.0	82.0
trnH-CAC-psbA_p2	CTGCCTTAATCCACTTGGCT	AGGTTACAGGTTTGGGCAAG	50.0	50.0	57.9	58.0	664-670 bp	0.75	70.1
trnH-CAC-psbA_p3	CTGCCTTAATCCACTTGGCT	GAAGGTTACAGGTTTGGGCA	50.0	50.0	57.9	58.0	666-672 bp	0.75	70.0
trnH-CAC-psbA_p4	CTGCCTTAATCCACTTGGCT	GGTTACAGGTTTGGGCAAGA	50.0	50.0	57.9	58.0	663-669 bp	0.75	70.0
trnH-CAC-psbA_p5	CTGCCTTAATCCACTTGGCT	TGAAGGTTACAGGTTTGGGC	50.0	50.0	57.9	58.0	667-668 bp	0.5	59.6