Hotspot

ycf1

SSC · 124620-130109 · 5490 bp

Back to Crowea

Pi 0.00194
MarkerSeek score 53.70
Primer available yes
Label ycf1

Feature Metadata

label_name
ycf1
feature_type
gene
region
SSC
length_bp
5490
variable_sites
16
pi
0.00194
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
53.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf1_p1 CCAAACATGGACATGGTCGA GGGGTTACTTTTTGGATAAAAACA 50.0 33.3 58.2 56.0 230 bp 1.0 40.7
ycf1_p2 TCCCAAACATGGACATGGTC GGGGTTACTTTTTGGATAAAAACA 50.0 33.3 57.8 56.0 232 bp 1.0 40.7
ycf1_p3 CCAAACATGGACATGGTCGA GGGGTTACTTTTTGGATAAAAACAA 50.0 32.0 58.2 56.7 230 bp 1.0 40.7
ycf1_p4 TCCCAAACATGGACATGGTC GGGGTTACTTTTTGGATAAAAACAA 50.0 32.0 57.8 56.7 232 bp 1.0 40.7
ycf1_p5 CCAAACATGGACATGGTCGA GGGGTTACTTTTTGGATAAAAAC 50.0 34.8 58.2 54.4 230 bp 1.0 40.7