Hotspot

rps16

LSC · 4736-5903 · 1168 bp

Back to Crowea

Pi 0.00229
MarkerSeek score 48.40
Primer available yes
Label rps16

Feature Metadata

label_name
rps16
feature_type
gene
region
LSC
length_bp
1168
variable_sites
4
pi
0.00229
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
48.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16_p1 ACTCCTCCACACCCTCTTTT GCAATGACTATTCACGATTCCA 50.0 40.9 58.2 56.9 1263-1271 bp 1.0 54.0
rps16_p2 ACTCCTCCACACCCTCTTTT AGCAATGACTATTCACGATTCCA 50.0 39.1 58.2 58.2 1264-1272 bp 1.0 53.3
rps16_p3 ACTCCTCCACACCCTCTTTT AGCAATGACTATTCACGATTCC 50.0 40.9 58.2 56.6 1264-1272 bp 1.0 51.8
rps16_p4 GACTCCTCCACACCCTCTTT GCAATGACTATTCACGATTCCA 55.0 40.9 58.6 56.9 1264-1272 bp 1.0 50.2
rps16_p5 ACTCCTCCACACCCTCTTTT GCAATGACTATTCACGATTCCAT 50.0 39.1 58.2 57.3 1263-1271 bp 1.0 49.7