Hotspot

psaI-ycf4

LSC · 60096-60539 · 444 bp

Back to Crowea

Pi 0.00603
MarkerSeek score 59.80
Primer available yes
Label psaI-ycf4

Feature Metadata

label_name
psaI-ycf4
feature_type
igs
region
LSC
length_bp
444
variable_sites
4
pi
0.00603
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaI-ycf4_p1 GCAATGGCAATGGCTTCTTT AAAAAGGATAAGGGCCCAGC 45.0 50.0 57.9 58.1 583-588 bp 1.0 78.9
psaI-ycf4_p2 GCAATGGCAATGGCTTCTTT TAAGGGCCCAGCAGAAATTG 45.0 50.0 57.9 58.1 575-580 bp 1.0 78.8
psaI-ycf4_p3 AGTATTTCCGGCAATGGCAA AAAAAGGATAAGGGCCCAGC 45.0 50.0 58.2 58.1 593-598 bp 1.0 78.5
psaI-ycf4_p4 AGTATTTCCGGCAATGGCAA TAAGGGCCCAGCAGAAATTG 45.0 50.0 58.2 58.1 585-590 bp 1.0 78.4
psaI-ycf4_p5 GGCCTAGTATTTCCGGCAAT AAAAAGGATAAGGGCCCAGC 50.0 50.0 57.7 58.1 598-603 bp 1.0 77.6