Hotspot

accD-psaI

LSC · 59174-59981 · 808 bp

Back to Crowea

Pi 0.00341
MarkerSeek score 64.00
Primer available yes
Label accD-psaI

Feature Metadata

label_name
accD-psaI
feature_type
igs
region
LSC
length_bp
808
variable_sites
4
pi
0.00341
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.97
markerseek_score
64.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD-psaI_p1 AGTTATTTCAGTTCCACGCCT AAAGAAGCCATTGCCATTGC 42.9 45.0 57.6 57.9 915-941 bp 1.0 70.5
accD-psaI_p2 TCCAATCGTACCACGTAATCC AAAGAAGCCATTGCCATTGC 47.6 45.0 57.6 57.9 956-982 bp 1.0 70.2
accD-psaI_p3 CCAATCGTACCACGTAATCCT AAAGAAGCCATTGCCATTGC 47.6 45.0 57.6 57.9 955-981 bp 1.0 70.2
accD-psaI_p4 AGTTATTTCAGTTCCACGCCT TTGCCATTGCCGGAAATACT 42.9 45.0 57.6 58.2 905-931 bp 1.0 70.1
accD-psaI_p5 TCCAATCGTACCACGTAATCC TTGCCATTGCCGGAAATACT 47.6 45.0 57.6 58.2 946-972 bp 1.0 69.8