Hotspot

rbcL-accD

LSC · 57109-57679 · 571 bp

Back to Crowea

Pi 0.00117
MarkerSeek score 58.60
Primer available yes
Label rbcL-accD

Feature Metadata

label_name
rbcL-accD
feature_type
igs
region
LSC
length_bp
571
variable_sites
1
pi
0.00117
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rbcL-accD_p1 GAGGCTAGCAAATGGAGTCC TCCCCTTTACCCTTACCCTT 55.0 50.0 58.0 57.2 713-714 bp 1.0 73.8
rbcL-accD_p2 GAGGCTAGCAAATGGAGTCC TTCCCCTTTACCCTTACCCT 55.0 50.0 58.0 57.2 714-715 bp 1.0 73.8
rbcL-accD_p3 GGAGGCTAGCAAATGGAGTC TCCCCTTTACCCTTACCCTT 55.0 50.0 58.0 57.2 714-715 bp 1.0 73.8
rbcL-accD_p4 GGAGGCTAGCAAATGGAGTC TTCCCCTTTACCCTTACCCT 55.0 50.0 58.0 57.2 715-716 bp 1.0 73.8
rbcL-accD_p5 TGCTCGCGAAGGTAATGAAA TCCCCTTTACCCTTACCCTT 45.0 50.0 57.9 57.2 741-742 bp 1.0 73.4