Hotspot

ndhC-trnV-UAC

LSC · 51396-51848 · 453 bp

Back to Crowea

Pi 0.00589
MarkerSeek score 42.70
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
453
variable_sites
4
pi
0.00589
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
42.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 GCGGAAATTCGAAATGCCAA AGAAGGTCTACGGTTCGAGT 45.0 50.0 58.0 57.8 572 bp 1.0 78.9
ndhC-trnV-UAC_p2 TCTGGCCCTTTGTTAATCGG AGAAGGTCTACGGTTCGAGT 50.0 50.0 57.9 57.8 602 bp 1.0 78.0
ndhC-trnV-UAC_p3 ACTGCGGAAATTCGAAATGC AGAAGGTCTACGGTTCGAGT 45.0 50.0 57.7 57.8 575 bp 1.0 76.9
ndhC-trnV-UAC_p4 TAATCGGGGCTAAAACTGCG AGAAGGTCTACGGTTCGAGT 50.0 50.0 58.3 57.8 589 bp 1.0 76.2
ndhC-trnV-UAC_p5 GCGGAAATTCGAAATGCCAA GAAGGTCTACGGTTCGAGTC 45.0 55.0 58.0 57.5 571 bp 1.0 76.1