Pi
0.00323
MarkerSeek score
60.80
Primer available
yes
Label
trnT-UGU-trnL-UAA
Feature Metadata
Pi Curve
Haplotype Network
Species Separation
Primer Ranking
| Primer ID | fwd_seq | rev_seq | fwd_gc | rev_gc | fwd_tm | rev_tm | amplicon_size | cross_species_success_rate | primer_score |
|---|---|---|---|---|---|---|---|---|---|
| trnT-UGU-trnL-UAA_p1 | CAACTAGAGAAAAGCCGGCT | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 57.9 | 58.0 | 1138-1145 bp | 1.0 | 79.5 |
| trnT-UGU-trnL-UAA_p2 | GCGATGCTCTAACCTCTGAG | ACCAATTTCGCCATATCCCC | 55.0 | 50.0 | 57.9 | 58.0 | 1083-1090 bp | 1.0 | 79.3 |
| trnT-UGU-trnL-UAA_p3 | CAACTAGAGAAAAGCCGGCT | TAGCGTCTACCAATTTCGCC | 50.0 | 50.0 | 57.9 | 58.1 | 1146-1153 bp | 1.0 | 79.0 |
| trnT-UGU-trnL-UAA_p4 | ACAACTAGAGAAAAGCCGGC | ACCAATTTCGCCATATCCCC | 50.0 | 50.0 | 58.2 | 58.0 | 1139-1146 bp | 1.0 | 78.8 |
| trnT-UGU-trnL-UAA_p5 | GCGATGCTCTAACCTCTGAG | TAGCGTCTACCAATTTCGCC | 55.0 | 50.0 | 57.9 | 58.1 | 1091-1098 bp | 1.0 | 78.7 |