Hotspot

trnT-GGU-psbD

LSC · 32265-33482 · 1218 bp

Back to Crowea

Pi 0.00168
MarkerSeek score 61.60
Primer available yes
Label trnT-GGU-psbD

Feature Metadata

label_name
trnT-GGU-psbD
feature_type
igs
region
LSC
length_bp
1218
variable_sites
3
pi
0.00168
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.98
markerseek_score
61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnT-GGU-psbD_p1 GTGGTAGAGTAACGCCATGG AAAAACGAATCGGTCCCTCC 55.0 50.0 58.1 58.2 1342-1366 bp 1.0 79.6
trnT-GGU-psbD_p2 TCAGTGGTAGAGTAACGCCA AAAAACGAATCGGTCCCTCC 50.0 50.0 58.1 58.2 1345-1369 bp 1.0 79.4
trnT-GGU-psbD_p3 TTGAACCAAAGGGCCCTTTT AAAAACGAATCGGTCCCTCC 45.0 50.0 58.1 58.2 1368-1392 bp 1.0 79.2
trnT-GGU-psbD_p4 AATTGAACCAAAGGGCCCTT AAAAACGAATCGGTCCCTCC 45.0 50.0 57.9 58.2 1370-1394 bp 1.0 79.2
trnT-GGU-psbD_p5 ATTGAACCAAAGGGCCCTTT AAAAACGAATCGGTCCCTCC 45.0 50.0 57.9 58.2 1369-1393 bp 1.0 79.2