Hotspot

rps16-trnQ-UUG

LSC · 5904-6701 · 798 bp

Back to Crowea

Pi 0.00426
MarkerSeek score 63.30
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
798
variable_sites
5
pi
0.00426
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.98
markerseek_score
63.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 855-871 bp 1.0 80.2
rps16-trnQ-UUG_p2 AAGTCGCACGTTGCTTTCTA CGAAATTGAAATGGGGCGTC 45.0 50.0 58.1 58.1 914-930 bp 1.0 80.0
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA GAAATTGAAATGGGGCGTCG 45.0 50.0 58.1 58.1 913-929 bp 1.0 80.0
rps16-trnQ-UUG_p4 TCATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 866-882 bp 1.0 79.7
rps16-trnQ-UUG_p5 TCATGTCCTTCAAGTCGCAC GAAATTGAAATGGGGCGTCG 50.0 50.0 58.2 58.1 924-940 bp 1.0 79.4