Hotspot

matK-trnK-UUU

LSC · 3600-4333 · 734 bp

Back to Crowea

Pi 0.00182
MarkerSeek score 59.70
Primer available yes
Label matK-trnK-UUU

Feature Metadata

label_name
matK-trnK-UUU
feature_type
igs
region
LSC
length_bp
734
variable_sites
2
pi
0.00182
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
59.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
matK-trnK-UUU_p1 TACTCCCGAAAAAGAAGCGG TGGGTTGCTAACTCAATGGT 50.0 45.0 57.9 57.0 851-853 bp 1.0 71.8
matK-trnK-UUU_p2 TACTCCCGAAAAAGAAGCGG TCAATGGTAGAGTACTCGGCT 50.0 47.6 57.9 58.0 839-841 bp 1.0 71.5
matK-trnK-UUU_p3 CGGGTATAGCAAGTCGTGTT TGGGTTGCTAACTCAATGGT 50.0 45.0 57.7 57.0 834-836 bp 1.0 70.2
matK-trnK-UUU_p4 AGCAAGTCGTGTTGTTGAGA TGGGTTGCTAACTCAATGGT 45.0 45.0 57.7 57.0 827-829 bp 1.0 70.1
matK-trnK-UUU_p5 CGGGTATAGCAAGTCGTGTT TCAATGGTAGAGTACTCGGCT 50.0 47.6 57.7 58.0 822-824 bp 1.0 69.9