Hotspot

trnH-GUG-psbA

LSC · 32-414 · 383 bp

Back to Crowea

Pi 0.0157
MarkerSeek score 57.50
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
383
variable_sites
9
pi
0.0157
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
57.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 CTTGATCCACTTGGCCACAT CGTGCTAACCTTGGTATGGA 50.0 50.0 58.2 57.3 504-505 bp 1.0 74.7
trnH-GUG-psbA_p2 CTTGATCCACTTGGCCACAT TCGTGCTAACCTTGGTATGG 50.0 50.0 58.2 57.3 505-506 bp 1.0 74.7
trnH-GUG-psbA_p3 TTGATCCACTTGGCCACATC CGTGCTAACCTTGGTATGGA 50.0 50.0 58.2 57.3 503-504 bp 1.0 74.7
trnH-GUG-psbA_p4 TTGATCCACTTGGCCACATC TCGTGCTAACCTTGGTATGG 50.0 50.0 58.2 57.3 504-505 bp 1.0 74.7
trnH-GUG-psbA_p5 CTTGATCCACTTGGCCACAT CGTGCTAACCTTGGTATGGAA 50.0 47.6 58.2 58.0 504-505 bp 1.0 72.0