Hotspot

rps15-ycf1

SSC · 136196-136571 · 376 bp

Back to Croton

Pi 0.0731
MarkerSeek score 77.40
Primer available yes
Label rps15-ycf1

Feature Metadata

label_name
rps15-ycf1
feature_type
igs
region
SSC
length_bp
376
variable_sites
75
pi
0.0731
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.91
markerseek_score
77.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-ycf1_p1 CGCCGTCTAGAGTAACGATG TTCTTTGGCCCCATTTTCGA 55.0 45.0 58.0 58.0 953-1012 bp 1.0 91.7
rps15-ycf1_p2 AGGTCTTTTCGCATAGCTGG TTCTTTGGCCCCATTTTCGA 50.0 45.0 58.0 58.0 879-938 bp 1.0 91.7
rps15-ycf1_p3 CGCCGTCTAGAGTAACGATG GTCGCTTCAGTATGGCAAGA 55.0 50.0 58.0 58.0 911 bp 0.166667 46.4
rps15-ycf1_p4 CGCCGTCTAGAGTAACGATG AGTCGCTTCAGTATGGCAAG 55.0 50.0 58.0 58.0 912 bp 0.166667 46.4
rps15-ycf1_p5 AGGTCTTTTCGCATAGCTGG GTCGCTTCAGTATGGCAAGA 50.0 50.0 58.0 58.0 837 bp 0.166667 46.3