Hotspot

ndhF-trnL-UAG

SSC · 124978-126599 · 1622 bp

Back to Croton

Pi 0.0706
MarkerSeek score 83.00
Primer available yes
Label ndhF-trnL-UAG

Feature Metadata

label_name
ndhF-trnL-UAG
feature_type
igs
region
SSC
length_bp
1622
variable_sites
242
pi
0.0706
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.91
markerseek_score
83.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-trnL-UAG_p1 TGAAGAGGAAATTGCGCTGA AGCGTGTCTACCGATTTCAC 45.0 50.0 57.8 58.0 2303-2704 bp 1.0 90.5
ndhF-trnL-UAG_p2 TGAAGAGGAAATTGCGCTGA CAGCGTGTCTACCGATTTCA 45.0 50.0 57.8 58.0 2304-2705 bp 1.0 90.5
ndhF-trnL-UAG_p3 GCAATAGGTCGTGTGAACCA AGCGTGTCTACCGATTTCAC 50.0 50.0 58.2 58.0 2075-2476 bp 1.0 90.4
ndhF-trnL-UAG_p4 GCAATAGGTCGTGTGAACCA CAGCGTGTCTACCGATTTCA 50.0 50.0 58.2 58.0 2076-2477 bp 1.0 90.4
ndhF-trnL-UAG_p5 TGCAATAGGTCGTGTGAACC AGCGTGTCTACCGATTTCAC 50.0 50.0 58.2 58.0 2125-2164 bp 0.333333 60.4