Hotspot

accD-psaI

LSC · 67458-67962 · 505 bp

Back to Croton

Pi 0.0907
MarkerSeek score 80.30
Primer available yes
Label accD-psaI

Feature Metadata

label_name
accD-psaI
feature_type
igs
region
LSC
length_bp
505
variable_sites
112
pi
0.0907
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.93
markerseek_score
80.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
accD-psaI_p1 GGTGACAGCCAGTTTTGGTA GTTTTTCGAGACCCCGCTAT 50.0 50.0 58.0 58.0 1369-1441 bp 1.0 91.6
accD-psaI_p2 GGTGACAGCCAGTTTTGGTA CCATTGCAATTGCCGGAAAT 50.0 45.0 58.0 58.0 799-863 bp 1.0 91.6
accD-psaI_p3 GGTACAGGTCAGCTAAACGG GTTTTTCGAGACCCCGCTAT 55.0 50.0 58.0 58.0 1679 bp 0.166667 46.5
accD-psaI_p4 GGTACAGGTCAGCTAAACGG CCATTGCAATTGCCGGAAAT 55.0 45.0 58.0 58.0 1109 bp 0.166667 46.4
accD-psaI_p5 GGTACAGGTCAGCTAAACGG TCGAGACCCCGCTATAAGTT 55.0 50.0 58.0 57.9 1674 bp 0.166667 46.1