Hotspot

trnF-GAA-ndhJ

LSC · 56397-57216 · 820 bp

Back to Croton

Pi 0.0361
MarkerSeek score 77.50
Primer available yes
Label trnF-GAA-ndhJ

Feature Metadata

label_name
trnF-GAA-ndhJ
feature_type
igs
region
LSC
length_bp
820
variable_sites
80
pi
0.0361
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.97
markerseek_score
77.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnF-GAA-ndhJ_p1 GGGATAGCTCAGCAGGTAGA ACTCTATCAACCGCCGTCTA 55.0 50.0 58.0 58.0 1543-1633 bp 1.0 89.1
trnF-GAA-ndhJ_p2 GGGATAGCTCAGCAGGTAGA TACTCTATCAACCGCCGTCT 55.0 50.0 58.0 58.0 1544-1634 bp 1.0 89.1
trnF-GAA-ndhJ_p3 GGGATAGCTCAGCAGGTAGA AAAGATGCAGGGTCGTTTGT 55.0 45.0 58.0 58.0 1335-1371 bp 0.833333 81.6
trnF-GAA-ndhJ_p4 CCTCGTGTCACCAGTTCAAA AAAGATGCAGGGTCGTTTGT 50.0 45.0 58.1 58.0 1299-1335 bp 0.833333 81.5
trnF-GAA-ndhJ_p5 AAAAGGATCGCTGGAATCCC AAAGATGCAGGGTCGTTTGT 50.0 45.0 57.9 58.0 1796 bp 0.166667 46.0