Hotspot

psaA-ycf3

LSC · 50132-50741 · 610 bp

Back to Croton

Pi 0.0324
MarkerSeek score 75.60
Primer available yes
Label psaA-ycf3

Feature Metadata

label_name
psaA-ycf3
feature_type
igs
region
LSC
length_bp
610
variable_sites
44
pi
0.0324
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.97
markerseek_score
75.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaA-ycf3_p1 GCCCATTCCTCGAAAGAAGT AGCGCAGAATTGGTTGAAGA 50.0 45.0 57.9 58.1 707-800 bp 1.0 87.0
psaA-ycf3_p2 GCCCATTCCTCGAAAGAAGT AAGATCACAAGGCGTTTCGA 50.0 45.0 57.9 57.8 691-784 bp 1.0 86.7
psaA-ycf3_p3 GCCCATTCCTCGAAAGAAGT AGATCACAAGGCGTTTCGAA 50.0 45.0 57.9 57.8 690-783 bp 1.0 86.7
psaA-ycf3_p4 GCCCATTCCTCGAAAGAAGT TGAAGCGCAGAATTGGTTGA 50.0 45.0 57.9 58.4 710-803 bp 1.0 84.7
psaA-ycf3_p5 GCCCATTCCTCGAAAGAAGT TTGAAGCGCAGAATTGGTTG 50.0 45.0 57.9 57.6 711-804 bp 1.0 84.5