Hotspot

psbZ-trnG-GCC

LSC · 43909-44652 · 744 bp

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Pi 0.0604
MarkerSeek score 81.20
Primer available yes
Label psbZ-trnG-GCC

Feature Metadata

label_name
psbZ-trnG-GCC
feature_type
igs
region
LSC
length_bp
744
variable_sites
103
pi
0.0604
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.92
markerseek_score
81.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-GCC_p1 CTTCTCCTGATGGTTGGTCG GGGTAGAGCAGTTTGGTAGC 55.0 55.0 58.0 58.0 917-1539 bp 1.0 91.7
psbZ-trnG-GCC_p2 CTTCTCCTGATGGTTGGTCG CGACGTTGTTTTTCGACTGG 55.0 50.0 58.0 58.0 1213-1835 bp 1.0 91.5
psbZ-trnG-GCC_p3 GCATGGCCATTTGTGAAGTC GGGTAGAGCAGTTTGGTAGC 50.0 55.0 58.0 58.0 1197-1825 bp 1.0 91.5
psbZ-trnG-GCC_p4 GCATGGCCATTTGTGAAGTC CGACGTTGTTTTTCGACTGG 50.0 50.0 58.0 58.0 1493-2121 bp 1.0 91.1
psbZ-trnG-GCC_p5 CAAGACCGGAGCTATCAACC CGACGTTGTTTTTCGACTGG 55.0 50.0 58.1 58.0 1709-2336 bp 1.0 90.6