Hotspot

trnE-UUC-trnT-GGU

LSC · 37164-38784 · 1621 bp

Back to Croton

Pi 0.0555
MarkerSeek score 78.30
Primer available yes
Label trnE-UUC-trnT-GGU

Feature Metadata

label_name
trnE-UUC-trnT-GGU
feature_type
igs
region
LSC
length_bp
1621
variable_sites
162
pi
0.0555
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.80
markerseek_score
78.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnT-GGU_p1 GCTGCCTCCTTGAAAGAGAG TCTACCACTGAGTTAAAAGGGC 55.0 45.5 58.3 57.7 1252-1920 bp 1.0 71.6
trnE-UUC-trnT-GGU_p2 TGTCCTGAACCACTAGACGA TCTACCACTGAGTTAAAAGGGC 50.0 45.5 57.7 57.7 1231-1899 bp 1.0 71.5
trnE-UUC-trnT-GGU_p3 CCTGAACCACTAGACGATGG TCTACCACTGAGTTAAAAGGGC 55.0 45.5 57.4 57.7 1228-1896 bp 1.0 69.1
trnE-UUC-trnT-GGU_p4 CTGAACCACTAGACGATGGG TCTACCACTGAGTTAAAAGGGC 55.0 45.5 57.4 57.7 1227-1895 bp 1.0 69.1
trnE-UUC-trnT-GGU_p5 GCTGCCTCCTTGAAAGAGAG CTACCACTGAGTTAAAAGGGC 55.0 47.6 58.3 56.3 1251-1919 bp 1.0 67.9