Hotspot

trnD-GUC-trnY-GUA

LSC · 36474-36947 · 474 bp

Back to Croton

Pi 0.0495
MarkerSeek score 82.20
Primer available yes
Label trnD-GUC-trnY-GUA

Feature Metadata

label_name
trnD-GUC-trnY-GUA
feature_type
igs
region
LSC
length_bp
474
variable_sites
48
pi
0.0495
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.96
markerseek_score
82.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnD-GUC-trnY-GUA_p1 CGGTGCTCTGACCAATTGAA CTGGTTCAAATCCAGCTCGG 50.0 55.0 58.5 58.9 467-528 bp 1.0 80.5
trnD-GUC-trnY-GUA_p2 CGGTGCTCTGACCAATTGAA TCTACGCTGGTTCAAATCCA 50.0 45.0 58.5 56.9 473-534 bp 1.0 78.5
trnD-GUC-trnY-GUA_p3 CGGTGCTCTGACCAATTGAA TGGTTCAAATCCAGCTCGG 50.0 52.6 58.5 57.8 466-527 bp 1.0 77.8
trnD-GUC-trnY-GUA_p4 CGGTGCTCTGACCAATTGAA GTCTACGCTGGTTCAAATCCA 50.0 47.6 58.5 58.3 474-535 bp 1.0 77.2
trnD-GUC-trnY-GUA_p5 GGTGCTCTGACCAATTGAAC CTGGTTCAAATCCAGCTCGG 50.0 55.0 57.0 58.9 466-527 bp 1.0 76.3