Hotspot

psbM-trnD-GUC

LSC · 34976-36399 · 1424 bp

Back to Croton

Pi 0.0534
MarkerSeek score 80.60
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
1424
variable_sites
152
pi
0.0534
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.83
markerseek_score
80.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 AGAATGAACAGTGCAGTAGC TTCAATTGGTCAGAGCACCG 45.0 50.0 55.8 58.5 1010-1535 bp 1.0 70.3
psbM-trnD-GUC_p2 AGAATGAACAGTGCAGTAGC GTTCAATTGGTCAGAGCACC 45.0 50.0 55.8 57.0 1011-1536 bp 1.0 66.2
psbM-trnD-GUC_p3 AGAATGAACAGTGCAGTAGC TCAATTGGTCAGAGCACCG 45.0 52.6 55.8 57.8 1009-1534 bp 1.0 64.3
psbM-trnD-GUC_p4 AGAATGAACAGTGCAGTAGC AGTTCAATTGGTCAGAGCACC 45.0 47.6 55.8 58.5 1012-1537 bp 1.0 62.2
psbM-trnD-GUC_p5 AGAATGAACAGTGCAGTAGC AATTGGTCAGAGCACCGC 45.0 55.6 55.8 58.0 1007-1532 bp 1.0 58.1