Hotspot

atpF-atpH

LSC · 16615-17232 · 618 bp

Back to Croton

Pi 0.0532
MarkerSeek score 83.40
Primer available yes
Label atpF-atpH

Feature Metadata

label_name
atpF-atpH
feature_type
igs
region
LSC
length_bp
618
variable_sites
68
pi
0.0532
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.80
markerseek_score
83.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
atpF-atpH_p1 ATTAAACCCGAAACTCCCGG GGCTGGTTGTAGCATTAGCA 50.0 50.0 57.9 58.2 549-774 bp 1.0 89.1
atpF-atpH_p2 ATTAAACCCGAAACTCCCGG TGGGCTGGTTGTAGCATTAG 50.0 50.0 57.9 57.6 551-776 bp 1.0 87.7
atpF-atpH_p3 CAAGGAAACGAACGCGAAAG GGCTGGTTGTAGCATTAGCA 50.0 50.0 58.3 58.2 704 bp 0.166667 41.9
atpF-atpH_p4 AAGGAAACGAACGCGAAAGA GGCTGGTTGTAGCATTAGCA 45.0 50.0 58.4 58.2 703 bp 0.166667 41.3
atpF-atpH_p5 AGGAAACGAACGCGAAAGAA GGCTGGTTGTAGCATTAGCA 45.0 50.0 58.4 58.2 702 bp 0.166667 41.3