Hotspot

trnR-UCU-atpA

LSC · 13301-13697 · 397 bp

Back to Croton

Pi 0.0687
MarkerSeek score 76.60
Primer available yes
Label trnR-UCU-atpA

Feature Metadata

label_name
trnR-UCU-atpA
feature_type
igs
region
LSC
length_bp
397
variable_sites
54
pi
0.0687
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.91
markerseek_score
76.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-UCU-atpA_p1 GGTTCAAATCCTATTGGACGC CGCTTTCTTCTTCAGGAACA 47.6 45.0 57.3 56.0 252-449 bp 1.0 62.5
trnR-UCU-atpA_p2 ACAAGAAAAGCGTCCATTGTC CGCTTTCTTCTTCAGGAACA 42.9 45.0 57.4 56.0 505 bp 0.166667 17.7
trnR-UCU-atpA_p3 GACAAGAAAAGCGTCCATTGT CGCTTTCTTCTTCAGGAACA 42.9 45.0 57.4 56.0 506 bp 0.166667 17.7
trnR-UCU-atpA_p4 ACAAGAAAAGCGTCCATTGTC CGCTTTCTTCTTCAGGAACAAG 42.9 45.5 57.4 57.9 505 bp 0.166667 16.5
trnR-UCU-atpA_p5 GACAAGAAAAGCGTCCATTGT CGCTTTCTTCTTCAGGAACAAG 42.9 45.5 57.4 57.9 506 bp 0.166667 16.5