Hotspot

trnG-UCC-trnR-UCU

LSC · 12342-13228 · 887 bp

Back to Croton

Pi 0.106
MarkerSeek score 84.30
Primer available yes
Label trnG-UCC-trnR-UCU

Feature Metadata

label_name
trnG-UCC-trnR-UCU
feature_type
igs
region
LSC
length_bp
887
variable_sites
197
pi
0.106
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.85
markerseek_score
84.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnR-UCU_p1 AGCCTTCCAAGCTAACGATG AGAAGACCTCTGTCCTATCCA 50.0 47.6 58.0 56.9 751-968 bp 1.0 75.3
trnG-UCC-trnR-UCU_p2 ACCCTTAGCCTTCCAAGCTA AGAAGACCTCTGTCCTATCCA 50.0 47.6 58.0 56.9 757-974 bp 1.0 75.3
trnG-UCC-trnR-UCU_p3 AGCCTTCCAAGCTAACGATG AGGTTTAGAAGACCTCTGTCCT 50.0 45.5 58.0 57.9 757-974 bp 1.0 74.7
trnG-UCC-trnR-UCU_p4 ACCCTTAGCCTTCCAAGCTA AGGTTTAGAAGACCTCTGTCCT 50.0 45.5 58.0 57.9 763-980 bp 1.0 74.7
trnG-UCC-trnR-UCU_p5 CTAACGATGCGGGTTCGATT AGAAGACCTCTGTCCTATCCA 50.0 47.6 58.4 56.9 740-957 bp 1.0 72.1