Hotspot

trnS-GCU-trnG-UCC

LSC · 10967-11548 · 582 bp

Back to Croton

Pi 0.0813
MarkerSeek score 83.10
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
582
variable_sites
106
pi
0.0813
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.97
markerseek_score
83.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 TAGCAATCCGACGCTTTAGG ACCACTAAACTATACCCGCT 50.0 45.0 57.8 55.6 392-858 bp 1.0 71.4
trnS-GCU-trnG-UCC_p2 AGCAATCCGACGCTTTAGG ACCACTAAACTATACCCGCT 52.6 45.0 57.9 55.6 391-857 bp 1.0 64.6
trnS-GCU-trnG-UCC_p3 GACGCTTTAGGCCACTCAG ACCACTAAACTATACCCGCT 57.9 45.0 58.2 55.6 383-849 bp 1.0 63.2
trnS-GCU-trnG-UCC_p4 TAGCAATCCGACGCTTTAGG TACCACTAAACTATACCCGCT 50.0 42.9 57.8 55.6 393-859 bp 1.0 63.0
trnS-GCU-trnG-UCC_p5 ACTCGTACAACGGATTAGCA ACCACTAAACTATACCCGCT 45.0 45.0 56.7 55.6 407-873 bp 1.0 62.7