Hotspot

trnK-UUU-rps16

LSC · 6472-7177 · 706 bp

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Pi 0.0538
MarkerSeek score 85.20
Primer available yes
Label trnK-UUU-rps16

Feature Metadata

label_name
trnK-UUU-rps16
feature_type
igs
region
LSC
length_bp
706
variable_sites
95
pi
0.0538
unique_haplotype_count
6
primer_available
yes
alignment_reliability
0.98
markerseek_score
85.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnK-UUU-rps16_p1 GTTAGCCGCACTTAAAAGCC TTCGCCTTAATCAAACACAA 50.0 35.0 57.7 53.7 661-1157 bp 1.0 55.5
trnK-UUU-rps16_p2 CCGCACTTAAAAGCCGAGTA TTCGCCTTAATCAAACACAA 50.0 35.0 58.3 53.7 656-1152 bp 1.0 55.3
trnK-UUU-rps16_p3 GTTAGCCGCACTTAAAAGCC TCGCCTTAATCAAACACAAT 50.0 35.0 57.7 53.5 660-1156 bp 1.0 53.6
trnK-UUU-rps16_p4 CCGCACTTAAAAGCCGAGTA TCGCCTTAATCAAACACAAT 50.0 35.0 58.3 53.5 655-1151 bp 1.0 53.4
trnK-UUU-rps16_p5 GTTAGCCGCACTTAAAAGCC TCGCCTTAATCAAACACAATT 50.0 33.3 57.7 54.4 660-1156 bp 1.0 52.7