Hotspot

ycf1

SSC · 108872-114184 · 5313 bp

Back to Crotalaria

Pi 0.0439

MarkerSeek score 70.30

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5313
variable_sites: 408
pi: 0.0439
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 70.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GGAACCGTCCAATGCGATAT	CCACGACAAGGTCCATTCAA	50.0	50.0	58.1	58.1	394-403 bp	1.0	87.2
ycf1_p2	GGAACCGTCCAATGCGATAT	GGCTTTTCTGGAACGAATGC	50.0	50.0	58.1	58.0	878-893 bp	1.0	86.7
ycf1_p3	GTGGGAAACGGCAGAAACTA	GGCTTTTCTGGAACGAATGC	50.0	50.0	58.1	58.0	1000-1006 bp	0.5	61.7
ycf1_p4	GTGGGAAACGGCAGAAACTA	CCACGACAAGGTCCATTCAA	50.0	50.0	58.1	58.1	516-522 bp	0.5	61.0
ycf1_p5	GTGTTTTTCGTACCCCTCCA	GGCTTTTCTGGAACGAATGC	50.0	50.0	57.7	58.0	769-775 bp	0.5	60.8