Hotspot

rps3-rps19

LSC · 83091-83415 · 325 bp

Back to Crotalaria

Pi 0.0497

MarkerSeek score 74.20

Primer available yes

Label rps3-rps19

Feature Metadata

label_name: rps3-rps19
feature_type: igs
region: LSC
length_bp: 325
variable_sites: 26
pi: 0.0497
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 74.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rps19_p1	ACGTCCGGCAATTTGTACTT	GCGCCGGAGTAAAAAGTAGA	45.0	50.0	58.1	58.0	1200-1246 bp	1.0	86.8
rps3-rps19_p2	GAAATGTTGAGTTTGCGGGG	GCGCCGGAGTAAAAAGTAGA	50.0	50.0	57.9	58.0	1037-1083 bp	0.75	76.8
rps3-rps19_p3	GAAATGTTGAGTTTGCGGGG	GATTGGCCATACCGTTGCTA	50.0	50.0	57.9	58.0	792-811 bp	0.75	76.6
rps3-rps19_p4	GAAATGTTGAGTTTGCGGGG	TTGGCCATACCGTTGCTATC	50.0	50.0	57.9	58.0	790-809 bp	0.75	76.6
rps3-rps19_p5	AACCCTCCCTTCTCTGATCC	GCGCCGGAGTAAAAAGTAGA	55.0	50.0	58.1	58.0	1254-1297 bp	0.75	75.7