Hotspot

rps11-rpl36

LSC · 78822-79110 · 289 bp

Back to Crotalaria

Pi 0.0456

MarkerSeek score 72.90

Primer available yes

Label rps11-rpl36

Feature Metadata

label_name: rps11-rpl36
feature_type: igs
region: LSC
length_bp: 289
variable_sites: 22
pi: 0.0456
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 72.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps11-rpl36_p1	ATGGTGTACCCCTTCGTGTA	GAAAGAATCGGCGGAGAAGT	50.0	50.0	58.1	58.0	1000-1019 bp	1.0	86.6
rps11-rpl36_p2	GAGACAGAATAAAGCGCCCA	GAAAGAATCGGCGGAGAAGT	50.0	50.0	58.0	58.0	1411 bp	0.25	49.7
rps11-rpl36_p3	TGGAGACAGAATAAAGCGCC	GAAAGAATCGGCGGAGAAGT	50.0	50.0	58.0	58.0	1413 bp	0.25	49.7
rps11-rpl36_p4	GAGACAGAATAAAGCGCCCA	GGGCCTCGACTCGTAAAATT	50.0	50.0	58.0	58.0	993 bp	0.25	49.6
rps11-rpl36_p5	TGGAGACAGAATAAAGCGCC	GGGCCTCGACTCGTAAAATT	50.0	50.0	58.0	58.0	995 bp	0.25	49.6