Hotspot

psbI-psbK

LSC · 54136-54624 · 489 bp

Back to Crotalaria

Pi 0.0362

MarkerSeek score 82.00

Primer available yes

Label psbI-psbK

Feature Metadata

label_name: psbI-psbK
feature_type: igs
region: LSC
length_bp: 489
variable_sites: 32
pi: 0.0362
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 82.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbI-psbK_p1	TCTTCACGTCCTGGATTACG	CTTTTGTTTGGCAAGCTGCT	50.0	45.0	57.1	58.1	619-631 bp	1.0	79.4
psbI-psbK_p2	TCTTCACGTCCTGGATTACG	GTTTGGCAAGCTGCTGTAAG	50.0	50.0	57.1	57.9	614-626 bp	1.0	79.4
psbI-psbK_p3	TCTTCACGTCCTGGATTACG	GCGAAATTACCCGAAGCCTA	50.0	50.0	57.1	58.1	701-713 bp	1.0	78.5
psbI-psbK_p4	TCTTCACGTCCTGGATTACG	TGGCAAGCTGCTGTAAGTTT	50.0	45.0	57.1	58.3	611-623 bp	1.0	77.4
psbI-psbK_p5	TCTTCACGTCCTGGATTACG	TTGGCAAGCTGCTGTAAGTT	50.0	45.0	57.1	58.3	612-624 bp	1.0	77.4