Hotspot

trnS-GGA-psbI

LSC · 53833-54024 · 192 bp

Back to Crotalaria

Pi 0.0362

MarkerSeek score 72.10

Primer available yes

Label trnS-GGA-psbI

Feature Metadata

label_name: trnS-GGA-psbI
feature_type: igs
region: LSC
length_bp: 192
variable_sites: 13
pi: 0.0362
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 72.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GGA-psbI_p1	GGCTTTTTGTTTACCGAGGG	CGTAATCCAGGACGTGAAGA	50.0	50.0	57.3	57.1	250-256 bp	1.0	72.8
trnS-GGA-psbI_p2	TTCGAATCCCTCTCTTTCCG	CGTAATCCAGGACGTGAAGA	50.0	50.0	57.0	57.1	230-236 bp	1.0	71.3
trnS-GGA-psbI_p3	GGCTTTTTGTTTACCGAGGG	CGTAATCCAGGACGTGAAGAA	50.0	47.6	57.3	57.8	250-256 bp	1.0	70.4
trnS-GGA-psbI_p4	GGGTTCGAATCCCTCTCTTT	CGTAATCCAGGACGTGAAGA	50.0	50.0	56.9	57.1	233-239 bp	1.0	70.3
trnS-GGA-psbI_p5	GGTTCGAATCCCTCTCTTTCC	CGTAATCCAGGACGTGAAGA	52.4	50.0	58.1	57.1	232-238 bp	1.0	70.2