Hotspot

atpH-atpI

LSC · 22211-23279 · 1069 bp

Back to Crotalaria

Pi 0.0332

MarkerSeek score 78.20

Primer available yes

Label atpH-atpI

Feature Metadata

label_name: atpH-atpI
feature_type: igs
region: LSC
length_bp: 1069
variable_sites: 60
pi: 0.0332
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 78.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpI_p1	ATAACGGAAGCGGCAGAAAT	CCGCGGCTTATATAGGTGAA	45.0	50.0	58.0	57.3	1120-1142 bp	1.0	80.8
atpH-atpI_p2	ATAACGGAAGCGGCAGAAAT	ACAAGCGGTATTCAAGCTCT	45.0	45.0	58.0	57.2	1157-1179 bp	1.0	80.3
atpH-atpI_p3	GTCCAATAGAAGCAAGCCCA	CCGCGGCTTATATAGGTGAA	50.0	50.0	57.9	57.3	1158-1180 bp	1.0	79.9
atpH-atpI_p4	AATAACGGAAGCGGCAGAAA	CCGCGGCTTATATAGGTGAA	45.0	50.0	58.2	57.3	1121-1143 bp	1.0	79.6
atpH-atpI_p5	GTCCAATAGAAGCAAGCCCA	ACAAGCGGTATTCAAGCTCT	50.0	45.0	57.9	57.2	1195-1217 bp	1.0	79.4