Hotspot

atpA-atpF

LSC · 20511-20595 · 85 bp

Back to Crotalaria

Pi 0.0667

MarkerSeek score 76.50

Primer available yes

Label atpA-atpF

Feature Metadata

label_name: atpA-atpF
feature_type: igs
region: LSC
length_bp: 85
variable_sites: 9
pi: 0.0667
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 76.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA-atpF_p1	CCGAGATTCCGAAGCTGAAA	AGTCGAAACGGAAGCAGATC	50.0	50.0	58.0	58.0	759-763 bp	1.0	82.2
atpA-atpF_p2	CTGACCACGCCCTATAGGTA	CGGTTTCGAGTGAATGGCTA	55.0	50.0	58.0	58.0	848-852 bp	1.0	82.1
atpA-atpF_p3	CTGACCACGCCCTATAGGTA	AGTCGAAACGGAAGCAGATC	55.0	50.0	58.0	58.0	867-871 bp	1.0	82.0
atpA-atpF_p4	CCGAGATTCCGAAGCTGAAA	ATACCGTGCGAAATCAACGA	50.0	45.0	58.0	58.0	1207-1210 bp	0.5	61.2
atpA-atpF_p5	CTGACCACGCCCTATAGGTA	ATACCGTGCGAAATCAACGA	55.0	45.0	58.0	58.0	1315-1318 bp	0.5	61.1