Hotspot

trnK-UUU-rbcL

LSC · 4345-5278 · 934 bp

Back to Crotalaria

Pi 0.051

MarkerSeek score 83.50

Primer available yes

Label trnK-UUU-rbcL

Feature Metadata

label_name: trnK-UUU-rbcL
feature_type: igs
region: LSC
length_bp: 934
variable_sites: 76
pi: 0.051
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 83.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnK-UUU-rbcL_p1	AGCCGAGTACTCTACCATTGA	TTTGAATTCCCAGCAATGGA	47.6	40.0	58.0	55.5	956-996 bp	1.0	62.0
trnK-UUU-rbcL_p2	AGCCGAGTACTCTACCATTGA	TGAATTCCCAGCAATGGATACT	47.6	40.9	58.0	57.5	954-994 bp	1.0	61.9
trnK-UUU-rbcL_p3	AGCCGAGTACTCTACCATTGA	TTGAATTCCCAGCAATGGAT	47.6	40.0	58.0	55.2	955-995 bp	1.0	60.1
trnK-UUU-rbcL_p4	GCCGAGTACTCTACCATTGAG	TTTGAATTCCCAGCAATGGA	52.4	40.0	57.6	55.5	955-995 bp	1.0	59.3
trnK-UUU-rbcL_p5	GCCGAGTACTCTACCATTGAG	TGAATTCCCAGCAATGGATACT	52.4	40.9	57.6	57.5	953-993 bp	1.0	59.2