Hotspot

trnH-GUG-psbA

LSC · 76-350 · 275 bp

Back to Crotalaria

Pi 0.0586
MarkerSeek score 74.00
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
275
variable_sites
26
pi
0.0586
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.86
markerseek_score
74.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG GGTATCTGGTTTACCGCGTT 55.0 50.0 58.1 58.0 476-520 bp 1.0 87.6
trnH-GUG-psbA_p2 CAATCCACTGCCTTGATCCA GGTATCTGGTTTACCGCGTT 50.0 50.0 57.9 58.0 490-534 bp 1.0 87.5
trnH-GUG-psbA_p3 AATCCACTGCCTTGATCCAC GGTATCTGGTTTACCGCGTT 50.0 50.0 57.9 58.0 489-533 bp 1.0 87.4
trnH-GUG-psbA_p4 ACAATCCACTGCCTTGATCC GGTATCTGGTTTACCGCGTT 50.0 50.0 57.9 58.0 491-535 bp 1.0 87.4
trnH-GUG-psbA_p5 GATCCACTTGGCTACATCCG CCAATTGGTCAGGGAAGCTT 55.0 50.0 58.1 58.1 836-876 bp 0.75 75.4