Hotspot

ndhD-ccsA

SSC · 121924-122128 · 205 bp

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Pi 0.0423

MarkerSeek score 75.80

Primer available yes

Label ndhD-ccsA

Feature Metadata

label_name: ndhD-ccsA
feature_type: igs
region: SSC
length_bp: 205
variable_sites: 13
pi: 0.0423
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 75.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhD-ccsA_p1	ATCCGGATTTCGTTCTCTCC	GCGCTTGTGGCTTCTATAGG	50.0	55.0	57.1	58.8	387-421 bp	1.0	69.2
ndhD-ccsA_p2	ATCCGGATTTCGTTCTCTCC	GGCGCTTGTGGCTTCTATAG	50.0	55.0	57.1	58.8	388-422 bp	1.0	69.2
ndhD-ccsA_p3	ATCCGGATTTCGTTCTCTCC	CGCTTGTGGCTTCTATAGGA	50.0	50.0	57.1	57.1	386-420 bp	1.0	68.3
ndhD-ccsA_p4	CCGGATTTCGTTCTCTCCCT	GCGCTTGTGGCTTCTATAGG	55.0	55.0	59.2	58.8	385-419 bp	1.0	67.0
ndhD-ccsA_p5	CCGGATTTCGTTCTCTCCCT	GGCGCTTGTGGCTTCTATAG	55.0	55.0	59.2	58.8	386-420 bp	1.0	67.0