Hotspot

ndhE-psaC

SSC · 119669-120053 · 385 bp

Back to Croomia

Pi 0.0202

MarkerSeek score 69.60

Primer available yes

Label ndhE-psaC

Feature Metadata

label_name: ndhE-psaC
feature_type: igs
region: SSC
length_bp: 385
variable_sites: 10
pi: 0.0202
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 69.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-psaC_p1	GGAAGCAGCTATTGGGCTAT	CTTAGCTTTACACCCGTCCC	50.0	55.0	57.4	58.0	540-587 bp	1.0	80.6
ndhE-psaC_p2	GGAAGCAGCTATTGGGCTAT	TTACACCCGTCCCAAGGTAT	50.0	50.0	57.4	58.0	533-580 bp	1.0	80.5
ndhE-psaC_p3	GGAAGCAGCTATTGGGCTAT	TGCTTAGCTTTACACCCGTC	50.0	50.0	57.4	57.9	542-589 bp	1.0	80.1
ndhE-psaC_p4	GGAAGCAGCTATTGGGCTAT	CAAAACATCCGTTGGGCAAG	50.0	50.0	57.4	58.2	507-554 bp	1.0	79.1
ndhE-psaC_p5	TGTTATAGCGATTGCAGCCG	CTTAGCTTTACACCCGTCCC	50.0	55.0	58.8	58.0	561-608 bp	1.0	78.9