Hotspot

accD-psaI

LSC · 57422-58099 · 678 bp

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Pi 0.00737

MarkerSeek score 66.90

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 678
variable_sites: 7
pi: 0.00737
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	AGTTCCACGGTTTCTTTCCC	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	650-792 bp	1.0	84.4
accD-psaI_p2	GTTCCACGGTTTCTTTCCCT	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	649-791 bp	1.0	84.4
accD-psaI_p3	CCACGGTTTCTTTCCCTTGA	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	646-788 bp	1.0	84.4
accD-psaI_p4	TCCACGGTTTCTTTCCCTTG	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.0	58.0	647-789 bp	1.0	84.4
accD-psaI_p5	GTTCCACGGTTTCTTTCCCT	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.0	57.9	655-797 bp	1.0	83.7