Hotspot

ndhC-trnV-UAC

LSC · 49645-50186 · 542 bp

Back to Croomia

Pi 0.00876

MarkerSeek score 65.00

Primer available yes

Label ndhC-trnV-UAC

Feature Metadata

label_name: ndhC-trnV-UAC
feature_type: igs
region: LSC
length_bp: 542
variable_sites: 7
pi: 0.00876
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 65.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhC-trnV-UAC_p1	CGGGGCTAAAACTCCAGAAA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.8	57.8	664-674 bp	1.0	79.0
ndhC-trnV-UAC_p2	GGTCCTTTACTAATCGGGGC	AGAAGGTCTACGGTTCGAGT	55.0	50.0	57.7	57.8	678-688 bp	1.0	78.5
ndhC-trnV-UAC_p3	ATCGGGGCTAAAACTCCAGA	AGAAGGTCTACGGTTCGAGT	50.0	50.0	58.4	57.8	666-676 bp	1.0	77.2
ndhC-trnV-UAC_p4	AATCGGGGCTAAAACTCCAG	AGAAGGTCTACGGTTCGAGT	50.0	50.0	57.6	57.8	667-677 bp	1.0	77.2
ndhC-trnV-UAC_p5	CGGGGCTAAAACTCCAGAAA	AGCCCAATGCAACAAAATGC	50.0	45.0	57.8	58.5	746-756 bp	1.0	76.6