Hotspot

trnF-GAA-ndhJ

LSC · 47263-47965 · 703 bp

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Pi 0.0109

MarkerSeek score 63.10

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 703
variable_sites: 11
pi: 0.0109
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	CTCCACTAGGATGATGCACG	TGAAAGTTGGATAGGCTGGC	55.0	50.0	57.9	57.9	874-879 bp	1.0	79.4
trnF-GAA-ndhJ_p2	GGAAATGGTCGGGATAGCTC	TGAAAGTTGGATAGGCTGGC	55.0	50.0	57.8	57.9	854-859 bp	1.0	79.2
trnF-GAA-ndhJ_p3	AATGGTCGGGATAGCTCAGT	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.2	57.9	851-856 bp	1.0	78.9
trnF-GAA-ndhJ_p4	ATGGTCGGGATAGCTCAGTT	TGAAAGTTGGATAGGCTGGC	50.0	50.0	58.2	57.9	850-855 bp	1.0	78.9
trnF-GAA-ndhJ_p5	CTCCACTAGGATGATGCACG	ATAGGCTGGCCCTTACGTAA	55.0	50.0	57.9	58.2	864-869 bp	1.0	78.8