Hotspot

trnS-UGA-lhbA

LSC · 34429-34776 · 348 bp

Back to Croomia

Pi 0.0192

MarkerSeek score 61.20

Primer available yes

Label trnS-UGA-lhbA

Feature Metadata

label_name: trnS-UGA-lhbA
feature_type: igs
region: LSC
length_bp: 348
variable_sites: 10
pi: 0.0192
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA-lhbA_p1	GTTTTCAAGACCGGGGCTAT	ACCAACCATCAGAAGAAGCA	50.0	45.0	57.9	57.0	489 bp	1.0	73.0
trnS-UGA-lhbA_p2	AGCAAAAGGAGAGAGAGGGA	ACCAACCATCAGAAGAAGCA	50.0	45.0	57.7	57.0	547 bp	1.0	71.1
trnS-UGA-lhbA_p3	GTTTTCAAGACCGGGGCTAT	GACCAACCATCAGAAGAAGCA	50.0	47.6	57.9	58.2	490 bp	1.0	70.9
trnS-UGA-lhbA_p4	GTTTTCAAGACCGGGGCTAT	TGACCAACCATCAGAAGAAGC	50.0	47.6	57.9	58.2	491 bp	1.0	70.9
trnS-UGA-lhbA_p5	GTTTTCAAGACCGGGGCTAT	ACCAACCATCAGAAGAAGCAA	50.0	42.9	57.9	57.8	489 bp	1.0	70.8