Hotspot

psbM-trnD-GUC

LSC · 28596-29392 · 797 bp

Back to Croomia

Pi 0.00669

MarkerSeek score 66.70

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 797
variable_sites: 8
pi: 0.00669
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGAATGAACAGTGCCGTAGC	TGCCATATGTCTCACTGGGA	50.0	50.0	58.3	58.1	820-937 bp	1.0	81.6
psbM-trnD-GUC_p2	AGAATGAACAGTGCCGTAGC	TTCAATTGGTCAGAGCACCG	50.0	50.0	58.3	58.5	794-911 bp	1.0	78.7
psbM-trnD-GUC_p3	AGAATGAACAGTGCCGTAGC	ATGCCATATGTCTCACTGGG	50.0	50.0	58.3	57.1	821-938 bp	1.0	75.0
psbM-trnD-GUC_p4	TAGAATGAACAGTGCCGTAGC	TGCCATATGTCTCACTGGGA	47.6	50.0	58.1	58.1	821-938 bp	1.0	74.8
psbM-trnD-GUC_p5	ACTAGAATGAACAGTGCCGT	TGCCATATGTCTCACTGGGA	45.0	50.0	56.9	58.1	823-940 bp	1.0	74.7