Hotspot

atpF-atpH

LSC · 12871-13048 · 178 bp

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Pi 0.0187

MarkerSeek score 65.10

Primer available yes

Label atpF-atpH

Feature Metadata

label_name: atpF-atpH
feature_type: igs
region: LSC
length_bp: 178
variable_sites: 5
pi: 0.0187
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpF-atpH_p1	TTAAACCCGAAACTCCCAGC	CGGACTAGTCGTGGCATTAG	50.0	55.0	58.1	57.9	295-603 bp	1.0	79.2
atpF-atpH_p2	TTAAACCCGAAACTCCCAGC	GGACTAGTCGTGGCATTAGC	50.0	55.0	58.1	58.1	294-602 bp	1.0	79.0
atpF-atpH_p3	TTAAACCCGAAACTCCCAGC	GCGGAGGGTAAAATACGAGG	50.0	55.0	58.1	58.1	360-668 bp	1.0	78.9
atpF-atpH_p4	TTAAACCCGAAACTCCCAGC	AGTCGTGGCATTAGCACTTT	50.0	45.0	58.1	57.8	289-597 bp	1.0	78.7
atpF-atpH_p5	TTAAACCCGAAACTCCCAGC	ACTAGTCGTGGCATTAGCAC	50.0	50.0	58.1	57.7	292-600 bp	1.0	77.8