Hotspot

rps15-ycf1

SSC · 120154-120596 · 443 bp

Back to Crocus

Pi 0.0166
MarkerSeek score 50.50
Primer available yes
Label rps15-ycf1

Feature Metadata

label_name
rps15-ycf1
feature_type
igs
region
SSC
length_bp
443
variable_sites
11
pi
0.0166
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps15-ycf1_p1 TCAACAGACCCACAGTTTTCT CGATAACGGCAGTCATTTCAG 42.9 47.6 57.4 57.4 566-567 bp 1.0 58.3
rps15-ycf1_p2 TCAACAGACCCACAGTTTTCT CGATAACGGCAGTCATTTCA 42.9 45.0 57.4 56.3 566-567 bp 1.0 57.0
rps15-ycf1_p3 ACAGACCCACAGTTTTCTTCT CGATAACGGCAGTCATTTCAG 42.9 47.6 57.1 57.4 563-564 bp 1.0 56.0
rps15-ycf1_p4 TCAACAGACCCACAGTTTTCTT CGATAACGGCAGTCATTTCAG 40.9 47.6 58.0 57.4 566-567 bp 1.0 55.2
rps15-ycf1_p5 TTCAACAGACCCACAGTTTTCT CGATAACGGCAGTCATTTCAG 40.9 47.6 58.0 57.4 567-568 bp 1.0 55.2