Hotspot

psaC-ndhE

SSC · 114239-114583 · 345 bp

Back to Crocus

Pi 0.0232
MarkerSeek score 48.60
Primer available yes
Label psaC-ndhE

Feature Metadata

label_name
psaC-ndhE
feature_type
igs
region
SSC
length_bp
345
variable_sites
12
pi
0.0232
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
48.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaC-ndhE_p1 CATAAATTTTTACTGAATGTGACA AGCTATTGTTTCGTCGATCCA 25.0 42.9 52.3 57.5 428-443 bp 1.0 44.9
psaC-ndhE_p2 TCATAAATTTTTACTGAATGTGAC AGCTATTGTTTCGTCGATCCA 25.0 42.9 52.1 57.5 429-444 bp 1.0 44.9
psaC-ndhE_p3 CATAAATTTTTACTGAATGTGACA GCTATTGTTTCGTCGATCCA 25.0 45.0 52.3 56.0 427-442 bp 1.0 44.9
psaC-ndhE_p4 CATAAATTTTTACTGAATGTGACA AGCTATTGTTTCGTCGATCCAT 25.0 40.9 52.3 57.9 428-443 bp 1.0 44.9
psaC-ndhE_p5 TCATAAATTTTTACTGAATGTGAC GCTATTGTTTCGTCGATCCA 25.0 45.0 52.1 56.0 428-443 bp 1.0 44.9