Hotspot

psbM-trnD-GUC

LSC · 27710-28803 · 1094 bp

Back to Crocus

Pi 0.0116
MarkerSeek score 44.10
Primer available yes
Label psbM-trnD-GUC

Feature Metadata

label_name
psbM-trnD-GUC
feature_type
igs
region
LSC
length_bp
1094
variable_sites
19
pi
0.0116
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
44.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbM-trnD-GUC_p1 GGAACTAAAATGAACAGTGCAGT TATGCCATACACCTTGCTGG 39.1 50.0 57.6 57.7 1239-1255 bp 1.0 51.0
psbM-trnD-GUC_p2 AAAATGAACAGTGCAGTAGC TATGCCATACACCTTGCTGG 40.0 50.0 54.5 57.7 1233-1249 bp 1.0 50.6
psbM-trnD-GUC_p3 GGAACTAAAATGAACAGTGCAGT CCATACACCTTGCTGGGATT 39.1 50.0 57.6 57.6 1235-1251 bp 1.0 50.2
psbM-trnD-GUC_p4 GGAACTAAAATGAACAGTGCAGT TTCAATTGGTCAGAGCACCG 39.1 50.0 57.6 58.5 1211-1227 bp 1.0 50.0
psbM-trnD-GUC_p5 GGAACTAAAATGAACAGTGCAGT CACCTTGCTGGGATTGTAGT 39.1 50.0 57.6 57.5 1230-1246 bp 1.0 49.8