Hotspot

rpoB-trnC-GCA

LSC · 25342-26261 · 920 bp

Back to Crocus

Pi 0.0109
MarkerSeek score 51.80
Primer available yes
Label rpoB-trnC-GCA

Feature Metadata

label_name
rpoB-trnC-GCA
feature_type
igs
region
LSC
length_bp
920
variable_sites
15
pi
0.0109
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
51.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpoB-trnC-GCA_p1 TCCCTCATTTCCATTCCGGA TGTTAATTAGGCGACACCCG 50.0 50.0 58.4 58.0 1023-1025 bp 1.0 78.0
rpoB-trnC-GCA_p2 TTCCCTCATTTCCATTCCGG TGTTAATTAGGCGACACCCG 50.0 50.0 57.6 58.0 1024-1026 bp 1.0 77.8
rpoB-trnC-GCA_p3 TCCCTCATTTCCATTCCGGA CCGGGGATAAAGGATTTGCA 50.0 50.0 58.4 57.9 995-997 bp 1.0 77.6
rpoB-trnC-GCA_p4 TCCCTCATTTCCATTCCGGA ACCGGGGATAAAGGATTTGC 50.0 50.0 58.4 57.9 996-998 bp 1.0 77.6
rpoB-trnC-GCA_p5 TTCCCTCATTTCCATTCCGG CCGGGGATAAAGGATTTGCA 50.0 50.0 57.6 57.9 996-998 bp 1.0 77.4