Hotspot

trnS-GCU-trnG-UCC

LSC · 7367-8138 · 772 bp

Back to Crocus

Pi 0.019
MarkerSeek score 50.90
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
772
variable_sites
22
pi
0.019
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
50.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 AATCTGCCGCTTTAGTCCAC AACCGAAAGACCCCTTAACT 50.0 45.0 58.3 56.4 878-964 bp 1.0 67.9
trnS-GCU-trnG-UCC_p2 CAATCTGCCGCTTTAGTCCA AACCGAAAGACCCCTTAACT 50.0 45.0 58.3 56.4 879-965 bp 1.0 67.9
trnS-GCU-trnG-UCC_p3 CGGAGAGAGAGGGATTCGAA AACCGAAAGACCCCTTAACT 55.0 45.0 58.3 56.4 932-1018 bp 1.0 67.3
trnS-GCU-trnG-UCC_p4 GCTTTAGTCCACTCAGCCAT AACCGAAAGACCCCTTAACT 50.0 45.0 57.6 56.4 870-956 bp 1.0 66.7
trnS-GCU-trnG-UCC_p5 AATCTGCCGCTTTAGTCCAC AAACCGAAAGACCCCTTAACT 50.0 42.9 58.3 57.1 879-965 bp 1.0 66.0