Hotspot

psbK-psbI

LSC · 6916-7074 · 159 bp

Back to Crocus

Pi 0.0252
MarkerSeek score 61.40
Primer available yes
Label psbK-psbI

Feature Metadata

label_name
psbK-psbI
feature_type
igs
region
LSC
length_bp
159
variable_sites
6
pi
0.0252
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
61.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbK-psbI_p1 GTTTGGCAAGCTGCTGTAAG TTACGTCCCGGATCGTTAGA 50.0 50.0 57.9 58.0 267-281 bp 1.0 85.0
psbK-psbI_p2 GTTTGGCAAGCTGCTGTAAG ACGTCCCGGATCGTTAGATA 50.0 50.0 57.9 57.7 265-279 bp 1.0 83.2
psbK-psbI_p3 GTTTGGCAAGCTGCTGTAAG TACGTCCCGGATCGTTAGAT 50.0 50.0 57.9 57.7 266-280 bp 1.0 83.2
psbK-psbI_p4 TGGCAAGCTGCTGTAAGTTT TTACGTCCCGGATCGTTAGA 45.0 50.0 58.3 58.0 264-278 bp 1.0 83.0
psbK-psbI_p5 TTGGCAAGCTGCTGTAAGTT TTACGTCCCGGATCGTTAGA 45.0 50.0 58.3 58.0 265-279 bp 1.0 83.0